Basic Virology. Martinez J. Hewlett

virus infection. (a) The first step ...Figure 14.8 (a) The replication of Sindbis virus genome, and generation of t...Figure 14.9 A schematic representation of the coronavirus virion. This is th...Figure 14.10 The replication cycle of a coronavirus. Replication is entirely...Figure 14.11 The approximately 25‐nm‐diameter icosahedral capsid of positive...Figure 14.12 Coupled transcription–translation of bacteriophage Qβ RNA resul...

      15 Chapter 15Figure 15.1 The vesicular stomatitis virus (VSV) virion. All rhabdoviruses h...Figure 15.2 The VSV replication cycle. (a) Early events in infection begin w...Figure 15.3 A higher‐resolution schematic of the generation of positive‐sens...Figure 15.4 The genetic map and virion structure of Sendai virus, a typical ...Figure 15.5 The structure of influenza virus A. The virion is about 120 nm i...Figure 15.6 An outline of the replication cycle of influenza. Following viru...Figure 15.7 Antigenic changes in the surface glycoproteins of influenza A vi...Figure 15.8 The bunyavirus virion. The three ribonucleoprotein (RNP) segment...Figure 15.9 The ambisense strategy of gene expression exhibited by some buny...Figure 15.10 The 60‐nm‐diameter human reovirus with its double shell. The 10...Figure 15.11 The reovirus replication cycle. Virus attachment is followed by...Figure 15.12 The potato spindle tuber viroid genome. Various pathogenic stra...Figure 15.13 Prion‐specific protein.

      16 Chapter 16Figure 16.1 Polyomavirus and the genetic and transcript map of SV40 virus. (...Figure 16.2 The replication cycle of SV40 virus in a permissive cell. The re...Figure 16.3 The replication of SV40 DNA. The closed circular DNA has no end ...Figure 16.4 Representation of the two steps in transformation of a nonpermis...Figure 16.5 The human papillomavirus 16 (HPV‐16) genome. The 7‐kb circular g...Figure 16.6 The formation of a wart by cell proliferation caused by infectio...Figure 16.7 The genetic and transcription map of the 30‐kb adenovirus genome...Figure 16.8 Adenovirus DNA replication. The 5′ ends of the viral genome have...Figure 16.9 The 5000‐nucleotide (nt) linear genome of adeno‐associated virus...Figure 16.10 The capsid structure and compressed genome of bacteriophage ΦX1...

      17 Chapter 17Figure 17.1 Electron micrograph of an enveloped HSV‐1 virion revealing speci...Figure 17.2 The HSV‐1 genetic and transcription map. Specific features of th...Figure 17.3 The programmed cascade of HSV transcription at different stages ...Figure 17.4 The entry of HSV‐1 into a cell for the initiation of infection. ...Figure 17.5 The HSV‐1 productive and latent infection cycles. In productive ...Figure 17.6 Replication and encapsidation of viral genomes. (a) HSV DNA init...Figure 17.7 Immune fluorescence analysis of the rearrangement of nuclear str...Figure 17.8 Maturation of the HSV capsid and its envelopment by tegument and...Figure 17.9 The “decision” made by HSV upon infection of epidermal tissue en...Figure 17.10 The expression of HSV transcripts during latent infection and r...Figure 17.11 The Epstein–Barr virus (EBV) genome and the latency transcripts...

      18 Chapter 18Figure 18.1 The vaccinia virus virion. The structure of poxviruses is the mo...Figure 18.2 The replication cycle of vaccinia virus. Following viral attachm...Figure 18.3 Replication of poxvirus DNA. The covalently closed, circular gen...Figure 18.4 The structure and genetic map of T7 bacteriophage. (a) The 40‐kb...Figure 18.5 The genetic map and structure of bacteriophage T4. By convention...Figure 18.6 Rolling circle replication and packaging of phage T4 DNA. The pr...Figure 18.7 Time of appearance of various functions encoded by T4 bacterioph...Figure 18.8 The assembly of T4 bacteriophage. Note that assembly of the phag...Figure 18.9 The bacteriophage λ genetic map. Specific clustered functions ar...Figure 18.10 The earliest events in the infection of a bacteria by phage λ. ...Figure 18.11 A phylogenetic tree of selected large‐DNA‐containing virus fami...

      19 Chapter 19Figure 19.1 The structures of an oncornavirus and a mature lentivirus. Virio...Figure 19.2 Genetic maps of various retroviruses. Specific examples are disc...Figure 19.3 The replication cycle of a typical retrovirus. (1) Adsorption an...Figure 19.4 The detailed mechanism for formation of retroviral cDNA from vir...Figure 19.5 Splicing patterns of various retrovirus RNAs to generate subgeno...Figure 19.6 Cell division and oncogenes. (a) In the normal cell, division is...Figure 19.7 The genomic structure of yeast Ty1. The similarity to a retrovir...

      20 Chapter 20Figure 20.1 The HIV‐1 genome, viral particle, and transcripts. The figure sh...Figure 20.2 HIV‐1 entry in detail. HIV‐1 binds cell surface CD4 via its surf...Figure 20.3 The HIV‐1 life cycle. After receptor‐mediated membrane fusion at...Figure 20.4 Vif and APOBEC action. In the absence of effective HIV‐1 Vif pro...Figure 20.5 Tat activation of HIV‐1 transcription. A tripartite complex cons...Figure 20.6 Rev binds to the Rev response element (RRE) to mediate nuclear e...Figure 20.7 HIV‐1 Vpu protein blocks the antiviral effect of tetherin. In th...Figure 20.8 Nef and Vpu downregulate CD4 expression and Nef downregulates MH...Figure 20.9 The pathogenesis of HIV infection leading to AIDS. Acute infecti...Figure 20.10 Triphasic decay of HIV‐1 in plasma following initiation of effe...

      21 Chapter 21Figure 21.1 A diagram of the virion structure and a genomic and genetic map ...Figure 21.2 The three RNAs of hepatitis delta virus found in infected liver ...Figure 21.3 The genome of cauliflower mosaic virus. The three breaks in the ...

      22 Chapter 22Figure 22.1 The impact of molecular understanding of viral and host genes on...Figure 22.2 Complementation. Neither of two mutant viruses shown can replica...Figure 22.3 Replica plating of virus plaques to distinguish genotypes produc...Figure 22.4 Mapping restriction endonuclease cleavage sites on a viral genom...Figure 22.5 Three widely used cloning plasmids that replicate in E. coli. Th...Figure 22.6 Isolation of a specific restriction fragment of viral DNA cloned...Figure 22.7 A togavirus expression vector. Semliki Forest virus (a togavirus...Figure 22.8 Directed mutagenesis of viral DNA. (a) Single‐stranded DNA conta...Figure 22.9 Generating and isolating recombinant viruses. (a) As outlined in...Figure 22.10 Generating a recombinant virus using CRISPR‐Cas gene editing. I...

      23 Chapter 23Figure 23.1 Construction of a transgenic mouse. The desired DNA (here, the h...Figure 23.2 Use of SCID‐hu mice to study HIV pathogenesis. Human thymus–live...Figure 23.3 Use of ß‐galactosidase (ß‐gal) as a report...Figure 23.4 (a) MRI images of a mouse infected with HSV‐1 10 days post infec...Figure 23.5 Use of a protein microarray to assay patient antibody reactivity...

      24 Chapter 24Figure 24.1 Growth of the genomic databases. Data displayed here are taken f...Figure 24.2 Results of a BLAST Search for a Nucleotide Sequence. (a) Initial...Figure 24.3 Random versus scale‐free networks. (a) A random network, in whic...Figure 24.4 Yeast two‐hybrid detection system. Transformation vectors are cr...Figure 24.5 Protein interaction map (PIM) for the round worm, Caenorhabditis...Figure 24.6 Host–virus protein interaction map of hepatitis C virus (HCV): t...

      25 Chapter 25Figure 25.1 Mortality from infectious diseases in the United States between ...

      Guide

      1  Cover Page

      2  Dedication

      3  Basic Virology

      4  Copyright

      5  Preface to the First Edition

      6  Preface to the Second Edition

      7  Preface to the Third Edition

      8  Preface to the Fourth Edition

      9  Acknowledgments

      10  Table of Contents

      11