Прочая образовательная литература
Различные книги в жанре Прочая образовательная литератураBiology in Space and Life on Earth
This concise yet comprehensive treatment of the effects of spaceflight on biological systems includes issues at the forefront of life sciences research, such as gravitational biology, immune system response, bone cell formation and the effects of radiation on biosystems. Edited by a leading specialist at the European Space Agency (ESA) with contributions by internationally renowned experts, the chapters are based on the latest space laboratory experiments, including those on SPACELAB, ISS, parabolic flights and unmanned research satellites. An indispensable source for biologists, medical researchers and astronautics experts alike. The results of Space flight experiments, ground controls and flight simulations pave the way for a better understanding of gravity reactions in various organisms down to molecular mechanisms. This publication marks also the beginning of a new Space flight era with the construction and exploitation of the International Space Station (ISS) which provides a platform for an in-depth continuation of experiments under weightlessness in Low Earth Orbit and beyond.
Expression and Analysis of Recombinant Ion Channels
Filling the gap created over the past five years, during which many new techniques have entered the market, this book is directed at both the new and the experienced ion channel researcher wishing to learn more about the considerations and methods for studying recombinant ion channels. These latest developments are covered here for the first time, contributed by editors and authors working for major pharmaceutical companies and who routinely apply these techniques in their daily work. The first three chapters cover the use of the Xenopus oocyte expression system for structure-function studies, from basic approaches for manipulating ion channel cDNAs to more specialized but powerful techniques. This is followed by reviews of strategies and methodologies available for expressing channels in mammalian cells and for their analysis by patch-clamp electrophysiology. Chapters 6 to 8 review the latest methodologies for ion channel drug discovery, including high throughput screening using fluorescence and luminescence, as well as automated planar array electrophysiology. The remaining two chapters focus on approaches for determining ion channel crystal structures and on computational approaches to understanding channel mechanisms at atomic resolution. Rather than provide detailed protocols, indicated by references in each chapter, the authors provide a comprehensive and easily accessible overview of the techniques involved, reviewing underlying principles and providing working guidelines as well as an understanding of the key theoretical and practical considerations associated with each topic. In each case, this practical advice is illustrated by real life examples, taken either from the author's own experience or from key examples in the literature, providing valuable practical hints not found elsewhere. The result is a compendium of practical ion channel information that will prove a valuable resource to academic and industrial workers alike.
Proteomic and Genomic Analysis of Cardiovascular Disease
This is the very first book to focus on this new approach that will eventually aid in developing new diagnostic markers and therapies for controlling and treating heart disease – the number-one killer in the industrialized world. Divided into two parts, the book describes not only the potentials, but also the limitations of these technologies. The editors, both well known within the scientific community, provide new insights into the biochemical and cellular mechanisms of cardiovascular disease, as well as covering the transition into clinical applications. In so doing, they highlight the various strategies and technical aspects so as to assist the growing number of researchers intending to utilize these approaches. The result is an excellent way of educating and informing graduate students, post-doctoral fellows as well as researchers in academia and industry about the latest developments in this area.
Analysing Gene Expression, A Handbook of Methods
This book combines the experience of 225 experts on 900 pages. Scientists worldwide are currently overwhelmed by the ever-increasing number and diversity of genome projects. This handbook is your guide through the jungle of new methods and techniques available to analyse gene expression – the first to provide such a broad view of the measurement of mRNA and protein expression in vitro, in situ and even in vivo. Despite this broad approach, detail is sufficient for you to grasp the principles behind each method. In each case, the authors weigh up the advantages and disadvantages, paying particular attention to the automated, high-throughput processing demanded by the biotech industry. Completely up to date, the book covers such ground-breaking methods such as DNA microarrays, serial analysis of gene expression, differential display, and identification of open reading frame expressed sequence tags. All the methods and necessary equipment are presented visually in more than 300 mainly colour illustrations to assist their step-by-step reproduction in your laboratory. Each chapter is rounded off with its own set of extensive references that provide access to detailed experimental protocols. In short, the bible of analysing gene expression.
Myopia and the Control of Eye Growth
Myopia, the most common disorder of the eye, affects 80% of the population of some countries. Although its basis remains uncertain, recent development of animal models have permitted tests of hypothesis as to myopia's origins. The symposium proceedings cover normal growth of the eye including the roles of peptide factors, metabolism and accomodation, investigation of the deficiencies that lead to myopia and other refractive disorders.
Epithelial Anion Transport in Health and Disease
Cl- absorption and HCO3- secretion are intimately associated processes vital to epithelial function, itself a key physiological activity. Until recently the transporters responsible remained obscure, but a breakthrough occurred with the discovery of the SLC26 transporters family. It is now clear that the SLC26 transporters have broad physiological functions since mutations in several members are linked to a variety of diseases. This book describes the properties of this family in detail, with contributions from the leading global researchers in the field. Complementary views from experts on other ion channels are offered in the discussions, which make fascinating reading. This family consists of at least 10 genes, each of which has several splice variants. Most members of the family are expressed in the luminal membrane of epithelial cells. Characterization of anion transport by three members has revealed that all function as Cl-/HCO3- exchangers, suggesting that SLC26 transporters are responsible for the luminal Cl-/HCO3- exchange activity. The SLC26 transporters are activated by the CF transmembrane conductance regulator and activate it in turn, leading to a model in which these molecules act together to mediate epithelial Cl- absorption and HCO3- secretion. The book includes chapters on the transport of other molecules by the SLC26 family, including oxalate in the kidney and sugars in cochlear hair cells amongst others. It also describes recent discoveries that most SLC26 transporters bind to scaffold proteins and that they all contain a conserved domain predicted to participate in protein-protein interactions. These suggest the SLC26 transporters exist in complexes with other Cl- and HCO3- transporters, and possibly other regulatory proteins. This book explores the functional role of these interactions, leading to better understanding of transepithelial fluid and electrolyte secretion and the diseases associated with it.
Integration von Advanced Control in der Prozessindustrie
Das erste Buch, das Rapid Control Prototyping zur zeitnahen und effizienten Umsetzung von Steuerungsverfahren fur verfahrenstechnische Anlagen beschreibt Aufgrund zunehmender Komplexitat verfahrenstechnischer Prozesse und steigender Qualitats-, Umwelt- und Rentabilitatsanforderungen kommt dem Einsatz intelligenter Verfahren der Automatisierungs- und Leittechnik eine standig wachsende Bedeutung zu. Unter den Verfahren des Advanced Control, d.h. den hoheren Regelungsmethoden, haben sich dabei insbesondere modellgestutzte pradiktive Regelungen in der Praxis bestens bewahrt. Die praktische Umsetzung, mit leittechnischen Methoden in die betrieblich Prozessebene vorzudringen, wird detailliert und anschaulich beschrieben. Durch den Einsatz des Rapid Control Prototyping wird es moglich, Verfahren des Advanced Control aus der Simulations- und Entwicklungsumgebung heraus schnell, kostengunstig und zuverlassig in den betrieblichen Einsatz zu bringen. Damit wird ein ein wesentlicher Beitrag zur Effizienz und Qualitatssicherung beim Systementwurf geleistet. Ein wesentlicher Aspekt ist die vollstandige und sichere Integration von Verfahren des Advanced Control in das Prozessleitsystem SIMATIC PCS7 auf der Feldebene Geeignete Einsatzgebiete sind nahezu alle Industriezweige – von der chemischen, pharmazeutischen, biotechnologischen Industrie bis zur Kunstoffindustrie.
Advanced Computer-Assisted Techniques in Drug Discovery
The use of powerful computers has revolutionized molecular design and drug discovery. Thoroughly researched and well-structured, this comprehensive handbook covers highly effective and efficient techniques in 3D-QSAR and advanced statistical analysis. The emphasis is on showing users how to apply these methods and avoid costly and time-consuming methodical errors. Topics covered include * combination of statistical methods and molecular modeling tools * rational use of databases * advanced statistical techniques * neural networks and expert systems in molecular design This book addresses the practitioner in industry and research, as well as the novice wishing to become acquainted with modern tools in medicinal chemistry.
Introduction to Contamination Control and Cleanroom Technology
Contamination control is being used by more and more industries where the highest level of cleanliness and hygiene is of vital importance. This book covers the basic principles of contamination control and cleanroom technology from a holistic point of view. It deals with cleanliness and hygiene and their effects on the outcome of a process, reflecting the latest results from both scientific and practical points of view. The following topics are covered: contaminants and how they are measured cleanrooms and clean zones cleaning and decontamination cleanroom clothing the impact of people on cleanliness. Intended as an introduction to the area of contamination control, the text is also an excellent source of knowledge for people with both theoretical and practical experience. The Swedish version has been used for a long time within the Nordic countries as a basic training textbook within the pharmaceutical, microelectronics, food and beverage, optics and many other industries.
Ligand Design for G Protein-coupled Receptors
G protein-coupled receptors (GPCRs) are one of the most important target classes in pharmacology and are the target of many blockbuster drugs. Yet only with the recent elucidation of the rhodopsin structure have these receptors become amenable to a rational drug design. Based on recent examples from academia and the pharmaceutical industry, this book demonstrates how to apply the whole range of bioinformatics, chemoinformatics and molecular modeling tools to the rational design of novel drugs targeting GPCRs. Essential reading for medicinal chemists and drug designers working with this largest class of drug targets in the human genome.