Molecular Biotechnology. Bernard R. Glick

       Reducing Acetate

       Bioreactors

       Typical Large-Scale Fermentation Systems

       Two-Stage Fermentation in Tandem Airlift Reactors

       Two-Stage Fermentation in a Single Stirred-Tank Reactor

       Batch versus Fed-Batch Fermentation

       Harvesting Microbial Cells

       Disrupting Microbial Cells

       Downstream Processing

       Protein Solubilization

       Utilizing an Immobilized Enzyme

       Magnetic Separation of Proteins

       Large-Scale Production of Plasmid DNA

       SUMMARY

       REFERENCES

       REVIEW QUESTIONS

       10 Genetic Engineering of Plants: Methodology

       Plant Transformation with the Ti Plasmid of A. Tumefaciens

       Ti Plasmid-Derived Vector Systems

       Microprojectile Bombardment

       Chloroplast Engineering

       Very High Level Protein Expression

       Use of Reporter Genes in Transformed Plant Cells

       Manipulation of Gene Expression in Plants

       Transient Gene Expression

       Plant Promoters

       Targeted Gene Editing

       Facilitating Protein Purification

       Protein Glycosylation

       Production of Marker-Free Transgenic Plants

       Removing Marker Genes from Nuclear DNA

       Removing Marker Genes from Chloroplast DNA

       SUMMARY

       REFERENCES

       REVIEW QUESTIONS

       11 Transgenic Plants

       Insect Resistance

       B. thuringiensis Insecticidal Toxin

       Increasing Expression of the B. thuringiensis Protoxin

       Other Strategies for Protecting Plants against Insects

       Preventing the Development of B. thuringiensis-Resistant Insects

       Targeting Aphids

       Virus Resistance

       Viral Coat Protein-Mediated Protection

       Protection by Expression of Other Genes

       Herbicide Resistance

       Fungus and Bacterium Resistance

       Salt and Drought Stress

       Fruit Ripening and Flower Wilting

       Modification of Plant Nutritional Content

       Amino Acids

       Lipids

       Vitamins

       Iron

       Gluten

       Modification of Food Plant Taste and Appearance

       Preventing Discoloration

       Starch

       Plants as Bioreactors

       Antibodies

       Poly(3-hydroxybutyric Acid)

       Eddible Vaccines

       Plant Yield

       Altering Lignin Content

       Increasing Oxygen Content

       SUMMARY

       REFERENCES

       REVIEW QUESTIONS

       12 Transgenic Animals

       Transgenic Mice: Methodology

       DNA Microinjection Method

       Retroviral Vector Method

       Engineered Embryonic Stem Cell Method

       Conditional Gene Inactivation with the Cre–loxP Recombination System

       Genome Editing with the CRISPR-Cas System

       Gene Knockdown by RNA Interference

       Transgenic Mice: Applications

       Transgenic Disease Models: Alzheimer Disease

       Transgenic Mice as Test Systems

       Control of Transgene Expression

       Conditional Control of Cell Death

       Transgenic Livestock

       Cloning Livestock by Somatic Cell Nuclear Transfer

       Production of Pharmaceuticals

       Production of Donor Organs

       Disease Resistant Livestock

       Improving Milk Quality

       Improving Animal Production Traits

       Transgenic Poultry

       Transgenic Fish

       SUMMARY

       REFERENCES

       REVIEW QUESTIONS

       13 Molecular Biotechnology and Society

       Development of Guidelines for Recombinant DNA Research

       Deliberate Release of Genetically Modified Microorganisms

       Environmental Concerns

       Regulations

       Regulation of Genetically Modified Foods

       Food Ingredients Produced by Genetically Engineered Microorganisms

       Genetically Modified Crops

       Genetically Engineered Livestock

       Societal Concerns About Genetically Modified Foods

       Alteration of Nutritional Content of Food

       Potential for Introducing Toxins or Allergens into Food

       Potential for Transferring Transgenes from Food to Humans or Intestinal Microorganisms

       Controversy About the Labeling of Genetically Modified Foods

       Impact of Genetically Engineered