Molecular Biotechnology. Bernard R. Glick

       DNase I

       Alginate Lyase

       Phenylalanine Ammonia Lyase

       α1-Antitrypsin

       Glycosidases

       Masking Nonhuman Epitopes

       Engineering Bacteriophages

       Targeting Mitochondria

       Lactic Acid Bacteria

       Interleukin-10

       Leptin

       An HIV Inhibitor

       Insulin

       Recombinant Antibodies

       Hybrid Human–Mouse Monoclonal Antibodies

       Human Monoclonal Antibodies

       Antibody Fragments

       Combinatorial Libraries of Antibody Fragments

       A Combinatorial Library of Full-Length Antibodies

       Shuffling CDR Sequences

       Dual-Variable-Domain Antibodies

       Anticancer Antibodies

       Antibodies Against Various Diseases

       Antiobesity Antibodies

       Enhanced Antibody Half-Life

       SUMMARY

       REFERENCES

       REVIEW QUESTIONS

       6 Nucleic Acids as Therapeutic Agents

       Targeting Specific mRNA and DNA Sequences

       Antisense RNA

       Aptamers

       Ribozymes and DNAzymes

       Interfering RNA

       Zinc Finger Nucleases

       CRISPR-Cas System

       Nanozymes

       Nanoparticles

       Viral Delivery Systems

       Nonviral Delivery Systems

       Direct Injection

       Lipids

       Bacteria

       Dendrimers

       Antibodies

       Aptamers

       Transposons

       Gene Therapy

       Prodrug Activation Therapy

       Promoterless Gene Targeting

       SUMMARY

       REFERENCES

       REVIEW QUESTIONS

       7 Vaccines

       Vaccination

       Current and Future Vaccines

       Subunit Vaccines

       Herpes Simplex Virus

       Cholera

       SARS

       Staphylococcus aureus

       Human Papillomavirus

       Foot-and-Mouth Virus

       Streptococcus

       Delivery

       Peptide Vaccines

       Malaria

       Genetic Immunization: DNA Vaccines

       Delivery

       Cancer

       Dental Caries

       Attenuated Vaccines

       Herpes Simplex Virus

       Cholera

       Salmonella Species

       Leishmania Species

       Vector Vaccines

       Vaccines Directed against Viruses

       Vaccines Directed against Bacteria

       Bacteria as Antigen Delivery Systems

       Monoclonal Antibody Passive Immunity

       Influenza Virus

       SUMMARY

       REFERENCES

       REVIEW QUESTIONS

       8 Industrial and Environmental Uses of Recombinant Microorganisms

       Restriction Endonucleases

       Small Biological Molecules

       L-Ascorbic Acid

       Indigo

       Amino Acids

       Lycopene

       Antibiotics

       Biopolymers

       Microbial Degradation of Xenobiotics

       Genetic Engineering of Biodegradative Pathways

       Utilization of Starch and Sugars

       Commercial Production of Fructose and Alcohol

       Increasing Alcohol Production

       Improving Fructose Production

       Utilization of Cellulose and Hemicellulose

       Lignocellulosics

       Cellulase Genes

       Direct Conversion of Biomass to Ethanol

       Zymomonas mobilis

       Lipids from Cyanobacteria

       Hydrogen Production

       SUMMARY

       REFERENCES

       REVIEW QUESTIONS

       9 Large-Scale Production of Proteins from Recombinant Microorganisms

       Principles of Microbial Growth

       Batch Fermentation

       Fed-Batch Fermentation

       Continuous Fermentation

       Maximizing The Efficiency of The Fermentation Process

       High-Density Cell Cultures

       Increasing Plasmid Stability

       Quiescent E. Coli Cells

       Protein Secretion