Molecular Biotechnology. Bernard R. Glick

Assembling Oligonucleotides into Genes

       Gene Synthesis by PCR

       DNA Sequencing Technologies

       Dideoxynucleotide Procedure

       Pyrosequencing

       Sequencing using Reversible Chain Terminators

       Sequencing by Single Molecule Synthesis

       Sequencing Whole Genomes

       Preparation of Genomic DNA Sequencing Libraries

       High-Throughput Next-Generation Sequencing Strategies

       Genome Sequence Assembly

       Sequencing Metagenomes

       Genomics

       Transcriptomics

       Proteomics

       Metabolomics

       SUMMARY

       REFERENCES

       REVIEW QUESTIONS

       3 Production of Recombinant Proteins

       Protein Production in Prokaryotic Hosts

       Regulation of Transcription

       Increasing Translation Efficiency

       Increasing Protein Stability

       Increasing Protein Secretion

       Facilitating Protein Purification

       DNA Integration into the Host Chromosome

       Heterologous Protein Production in Eukaryotic Cells

       Posttranslational Modification of Eukaryotic Proteins

       General Features of Eukaryotic Expression Systems

       Yeast Expression Systems

       Baculovirus–Insect Cell Expression Systems

       Mammalian Cell Expression Systems

       Protein Engineering

       Directed Mutagenesis

       Random Mutagenesis

       Examples of Protein Engineering

       SUMMARY

       REFERENCES

       REVIEW QUESTIONS

       4 Molecular Diagnostics

       Immunological Approaches to Detect Protein Biomarkers

       Antibodies

       Agglutination

       Enzyme-Linked Immunosorbent Assays

       Protein Arrays to Detect Polygenic Diseases

       Immunoassays for Protein Conformation-Specific Disorders

       DNA-Based Diagnostic Approaches

       Hybridization Probes

       PCR-Based Detection Methods

       DNA Microarrays

       Whole Genome Sequencing to Assess Genetic Disease Risk

       Detecting RNA Signatures of Disease

       Detection of Disease-Associated Changes in Gene Expression Using Microarrays

       Detection of RNA Signatures of Antibiotic Resistance in Bacteria

       Detection of miRNA Signatures of Cancers

       Biofluorescent and Bioluminescent Systems

       Fluorescent Proteins

       Luciferase

       Microbial Biosensors

       SUMMARY

       REFERENCES

       REVIEW QUESTIONS

       5 Protein Therapeutics

       Pharmaceuticals

       Human Interferons

       Human Growth Hormone

       Tumor Necrosis Factor Alpha

       Extending Protein Half-Life

       Enzymes