An Introduction to Molecular Biotechnology. Группа авторов

Chapter 12 Gel and capillary electrophoresis Chapter 10 Next generation sequencing Chapter 14 Microsatellite analysis Chapter 11 SNP analysis Chapters 14 and 21 FISH Chapter 11 In situ hybridization Chapter 11 RNA (transcriptomics) RNA‐seq (NGS) Chapters 14 and 21 DNA microarrays Chapter 11 In situ hybridization Chapter 11 Cell and tissue culture Cells with reporter genes Cell sorting Organoid cultures Stem cells Cancer cells Hybridoma cells for production of monoclonal antibodies Cell cycle analysis Chapter 18 Patch clamp recording Chapter 17 Microscopy Light microscope (bright field, dark field, phase contrast, differential interference contrast) Chapter 19 Fluorescence microscope (confocal) Chapters 19 and 20 Immunofluorescence and GFP fusion proteins Chapter 19 Super‐resolution microscopy (STED, SIM, PALM, STORM) Chapter 19 Atomic force microscopy Chapter 19 Electron microscope Chapter 19 Scanning electron microscope (SEM) Cryoelectron microscopy Chapter 19 Image processing Cloning and expression Plasmid and viral vectors Chapter 15 Expression vectors Chapters 15 and 16 Fermenters Genomic and cDNA libraries Chapter 21 Reverse genetics Genetic engineering Transformation Chapter 15 Transfection Chapter 15 RNAi CRISPR–Cas gene editing Transgenic organism