Pathology of Genetically Engineered and Other Mutant Mice. Группа авторов

in Mice

Spontaneous mutations have traditionally been named for the readily observable phenotype(s) they initially present. In the pre‐genomic era chromosomes were defined in large part by the linkage maps of phenotypic mutants. The gene(s) impacted by a spontaneous mutation are not initially known, and the symbol for such a heritable phenotypic marker is italicized and all lower case for recessive mutations, such as bg for beige, ob for obese, and d for dilute. The first letter of the symbol is in upper case for dominant or semi‐dominant mutations, such as Tr for trembler. When the impacted gene is identified, if the mutation is a molecular lesion in only one gene, then the original marker symbol becomes a superscript of that gene for the allele symbol, Lyst^bg, Lep^ob, Myo5a^d, and Pmp22^Tr, respectively. Although gene symbols may change over time as the function and ontology of the gene is better understood, other than becoming a superscript of the gene, the allele symbol remains the same as does the allele name. For example the commonly used nude mouse gene name has changed considerably over time (Table 3.11). For mice, the gene symbol has the first letter capitalized and subsequent letters in lower case, all in italics. Hyphens are avoided except to depict new alleles of the initial phenotypic mutation (Table 3.12). For example, while interleukin 10 is commonly written in journals as IL‐10, the correct gene symbol would be Il10 and protein symbol would be IL10. Human gene symbols are essentially the same as for mice except the human gene symbols are all in capital letters and italicized. Greek letters are not used at all, only Roman letters and Arabic numbers. For example, interferon gamma is Ifng. The protein coded for by this gene is listed in capital letters with no italics (IFNG) for both mouse and human proteins.

Non‐engineered Induced Mutations

      The system for naming chemically or radiation‐induced mutations is similar to that for spontaneous mutations, except that while many are initially assigned phenotypic names and corresponding symbols, some are instead assigned program accession numbers. One example of this is nmf12, which was the 12th characterized ENU induced mutation in the neuroscience mutagenesis facility (nmf) at The Jackson Laboratory. Once the underlying mutation was found to be a H716R point substitution in the gene MER proto‐oncogene tyrosine kinase, the symbol changed to Mertk^nmf12 [40].

Table 3.11 Evolution of the nomenclature for nude.

nu original mutation symbol D11Bhm185e, Hfh11, whn‐ early gene symbols Foxn1 (current gene symbol, forkhead box N1) Foxn1 nu current allele symbol for the nude allelic mutation

Table 3.12 Nomenclature for spontaneous mouse mutations.

C57BL/6J‐Lystbg‐J/J C57BL/6J‐Pax3Sp‐d/JC57BL/6J = Inbred strain carrying the mutationLyst = Lysosomal trafficking regulator (gene name, italics)bg‐J = Allele symbol for beige Jackson. Note the first letter in the allele (bg‐J) is lower case indicating it is recessive

C57BL/6J‐Pax3Sp‐d/JC57BL/6J = Inbred strain carrying the mutationPax3 = Paired box 3 (gene name, italics)Sp‐d = Allele symbol for delayed splotch. Note the first letter in the allele (Sp‐d) is upper case indicating dominant or semi‐dominant

      Radiation‐induced mutations have also often been assigned phenotypic names, such as disproportionate micromelia (Col2a1^Dmm), but because radiation‐induced mutations often impact more than one gene, the mutation symbol is in those instances not superscripted to a single gene symbol. A.G. Searle reported several phenotypic mutants generated in neutron irradiation experiments, one of which caused severe ocular colobomas in heterozygotes and was therefore named coloboma, with the symbol Cm [41, 42]. When this mutation was determined to be a multigenic deletion encompassing 29 genes the symbol remained Cm but the name changed from coloboma to coloboma deletion region. The symbol and name could have been changed to follow chromosomal aberration nomenclature for deletions, but because of the long history of this mutation in the literature this was not done.

Chromosomal Aberration Nomenclature

      Mutations that include more than one gene or alter chromosomal organization are often, but not always, assigned symbols and names that indicate this. The symbol begins with an indicator of the nature of the mutation followed by details about the chromosomal location of the mutation in parentheses followed by a line number and lab code of the laboratory that identified it. The most common types of chromosomal aberrations are multigenic deletions, but we include here a short list of examples of a few other important types:

       Del(10)77H: Deletion in Chromosome 10 that was the 77th deletion identified at Harwell (http://www.informatics.jax.org/accession/MGI:5314347).

       Del(5Letm1‐Htt)1Jcs: Deletion in Chromosome 5 from the gene Letm1 to the gene Htt that was the first made by Dr. John Schimenti (http://www.informatics.jax.org/accession/MGI:3798057).

       Dp(7Sult1a1‐Spn)7Yah: Duplication in Chromosome 7 from the gene Sult1a1 to the gene Spn that was the seventh duplication made by Dr. Yann Herault (http://www.informatics.jax.org/accession/MGI:6342919).

       In(5)2Rk: Inversion in Chromosome 5 that was the 5th inversion generated by Dr. Thomas Roderick (http://www.informatics.jax.org/accession/MGI:3789592).

       Rb(11.16)2H: Robertsonian translocation between Chromosomes 11 and 16 that was the second Robertsonian translocation generated at Harwell (http://www.informatics.jax.org/accession/MGI:104106).

       T(12;16)1Cje: Reciprocal translocation between Chromosomes 12 and 16 that was the first reciprocal translocation generated by Dr. Charles Epstein (http://www.informatics.jax.org/accession/MGI:1860491).

      While chromosomal aberrations were traditionally generated primarily through radiation experiments, modern genetic engineering techniques permit more engineered strategies [43]. Transgenic insertions are also a form of chromosomal aberration, and they are covered in the “Genetically Engineered Mice” section.

Genetically Engineered Mice

      There are three basic types of genetically engineered mice: transgenic, targeted, and endonuclease‐mediated. Different nomenclature is used to distinguish these.

      Transgenic mice can be created to overexpress a gene, express a gene not normally found in mice (green fluorescent protein [GFP] from jellyfish), conditionally express genes, and many other engineered approaches not found normally in nature.

Transgenic nomenclature can be complex, especially as some transgenes still use older formats (Table 3.13). All transgenes begin with Tg followed by information in parentheses then a founder line number and lab code. If a single gene driven by its own promoter is inserted, then the information in the parentheses is simply that gene symbol, not italicized. The information in the parentheses