Прочая образовательная литература
Различные книги в жанре Прочая образовательная литератураMicrosystem Engineering of Lab-on-a-chip Devices
Written by an interdisciplinary team of chemists, biologists and engineers from one of the leading European centers for microsystem research, MIC in Lyngby, Denmark, this book introduces and discusses the different aspects of (bio)chemical microsystem development. Unlike other, far more voluminous and theoretical books on this topic, this is a concise, practical handbook, dealing with analytical applications, particularly in the life sciences. Topics include: * microfluidics * silicon micromachining * glass and polymer micromachining * packaging * analytical chemistry illustrated with examples taken mainly from ongoing research projects at MIC.
Protein Sequencing and Identification Using Tandem Mass Spectrometry
How to design, execute, and interpret experiments for protein sequencing using mass spectrometry The rapid expansion of searchable protein and DNA databases in recent years has triggered an explosive growth in the application of mass spectrometry to protein sequencing. This timely and authoritative book provides professionals and scientists in biotechnology research with complete coverage of procedures for analyzing protein sequences by mass spectrometry, including step-by-step guidelines for sample preparation, analysis, and data interpretation. Michael Kinter and Nicholas Sherman present their own high-quality, laboratory-tested protocols for the analysis of a wide variety of samples, demonstrating how to carry out specific experiments and obtain fast, reliable results with a 99% success rate. Readers will get sufficient experimental detail to apply in their own laboratories, learn about the proper selection and operation of instruments, and gain essential insight into the fundamental principles of mass spectrometry and protein sequencing. Coverage includes: Peptide fragmentation and interpretation of product ion spectra Basic polyacrylamide gel electrophoresis Preparation of protein digests for sequencing experiments Mass spectrometric analysis using capillary liquid chromatography Techniques for protein identification by database searches Characterization of modified peptides using tandem mass spectrometry And much more
Bioanalytics
Analytical methods are the essential enabling tools of the modern biosciences. This book presents a comprehensive introduction into these analytical methods, including their physical and chemical backgrounds, as well as a discussion of the strengths and weakness of each method. It covers all major techniques for the determination and experimental analysis of biological macromolecules, including proteins, carbohydrates, lipids and nucleic acids. The presentation includes frequent cross-references in order to highlight the many connections between different techniques. The book provides a bird´s eye view of the entire subject and enables the reader to select the most appropriate method for any given bioanalytical challenge. This makes the book a handy resource for students and researchers in setting up and evaluating experimental research. The depth of the analysis and the comprehensive nature of the coverage mean that there is also a great deal of new material, even for experienced experimentalists. The following techniques are covered in detail: – Purification and determination of proteins – Measuring enzymatic activity – Microcalorimetry – Immunoassays, affinity chromatography and other immunological methods – Cross-linking, cleavage, and chemical modification of proteins – Light microscopy, electron microscopy and atomic force microscopy – Chromatographic and electrophoretic techniques – Protein sequence and composition analysis – Mass spectrometry methods – Measuring protein-protein interactions – Biosensors – NMR and EPR of biomolecules – Electron microscopy and X-ray structure analysis – Carbohydrate and lipid analysis – Analysis of posttranslational modifications – Isolation and determination of nucleic acids – DNA hybridization techniques – Polymerase chain reaction techniques – DNA sequence and epigenetic modification analysis – Analysis of protein-nucleic acid interactions – Analysis of sequence data – Proteomics, metabolomics, peptidomics and toponomics – Chemical biology
X-Ray Crystallography of Biomacromolecules
Written by one of the most significant contributors to the progress of protein crystallography, this practical guide contains case studies, a troubleshooting section and pointers on data interpretation. It covers the theory, practice and latest achievements in x-ray crystallography, such that any researcher in structural biology will benefit from this extremely clearly written book. Part A covers the theoretical basis and such experimental techniques as principles of x-ray diffraction, solutions for the phase problem and time-resolved x-ray crystallography. Part B includes case studies for different kinds of x-ray crystal structure determination, such as the MIRAS and MAD techniques, molecular replacement, and the difference Fourier technique.
The Epigenome
This is the first book that describes the role of the Epigenome (cytosine methylation) in the interplay between nature and nurture. It focuses and stimulates interest in what will be one of the most exciting areas of post-sequencing genome science: the relationship between genetics and the environment. Written by the most reputable authors in the field, this book is essential reading for researchers interested in the science arising from the human genome sequence and its implications on health care, industry and society.
Acoustic Emission and Durability of Composite Materials
In this book, two kinds of analysis based on acoustic emission recorded during mechanical tests are investigated. In the first, individual, analysis, acoustic signature of each damage mechanism is characterized. So with a clustering method, AE signals that have similar shapes or similar features can be group together into a cluster. Afterwards, each cluster can be linked with a main damage. The second analysis is based on a global AE analysis, on the investigation of liberated energy, with a view to identify a critical point. So beyond this characteristic point, the criticality can be modeled with a power-law in order to evaluate time to failure.
Materials, Chemicals and Methods for Dental Applications
This book focuses on the materials used for dental applications looking at the fundamental issues and the developments that have taken place the past decade. While it provides a broad overview of dental materials, the chemicals that are used for the preparation and fabrication of dental materials are explained as well. Also, the desired properties of these materials are discussed and the relevance of the chemical, physical, and mechanical properties is elucidated. Methods for the characterization and classification, as well as clinical studies are reviewed here. In particular, materials for dental crowns, implants, toothpaste compositions, mouth rinses, as well as materials for toothbrushes and dental floss are discussed. For example, in toothpaste compositions, several classes of materials an chemcials are incorporated, such as abrasives, detergents, humectants, thickeners, sweeteners, coloring agents, bad breath reduction agents, flavoring agents, tartar control agents, and others. These chemicals, together with their structures, are detailed in the text.
Mechanical Behaviour of Organic Matrix Composites
The book focuses on the effect of ageing (thermo-oxidation, humid ageing) on the mechanical properties of organic matrix composite materials, covering: Bibliographic issues and a detailed state-of-the-art; phenomenological and experimental issues; modelling issues and models parameter identification; illustration and interpretation of experimental tests and proposal for novel test design in the light of the model predictions.
Biomedical Engineering Challenges
An important resource that puts the focus on the chemical engineering aspects of biomedical engineering In the past 50 years remarkable achievements have been advanced in the fields of biomedical and chemical engineering. With contributions from leading chemical engineers, Biomedical Engineering Challenges reviews the recent research and discovery that sits at the interface of engineering and biology. The authors explore the principles and practices that are applied to the ever-expanding array of such new areas as gene-therapy delivery, biosensor design, and the development of improved therapeutic compounds, imaging agents, and drug delivery vehicles. Filled with illustrative case studies, this important resource examines such important work as methods of growing human cells and tissues outside the body in order to repair or replace damaged tissues. In addition, the text covers a range of topics including the challenges faced with developing artificial lungs, kidneys, and livers; advances in 3D cell culture systems; and chemical reaction methodologies for biomedical imagining analysis. This vital resource: Covers interdisciplinary research at the interface between chemical engineering, biology, and chemistry Provides a series of valuable case studies describing current themes in biomedical engineering Explores chemical engineering principles such as mass transfer, bioreactor technologies as applied to problems such as cell culture, tissue engineering, and biomedical imaging Written from the point of view of chemical engineers, this authoritative guide offers a broad-ranging but concise overview of research at the interface of chemical engineering and biology.
Process Architecture in Biomanufacturing Facility Design
Essential information for architects, designers, engineers, equipment suppliers, and other professionals who are working in or entering the biopharmaceutical manufacturing field Biomanufacturing facilities that are designed and built today are radically different than in the past. The vital information and knowledge needed to design and construct these increasingly sophisticated biopharmaceutical manufacturing facilities is difficult to find in published literature—and it’s rarely taught in architecture or design schools. This is the first book for architects and designers that fills this void. Process Architecture in Biomanufacturing Facility Design provides information on design principles of biopharmaceutical manufacturing facilities that support emerging innovative processes and technologies, use state-of-the-art equipment, are energy efficient and sustainable, and meet regulatory requirements. Relying on their many years of hands-on design and operations experience, the authors emphasize concepts and practical approaches toward design, construction, and operation of biomanufacturing facilities, including product-process-facility relationships, closed systems and single use equipment, aseptic manufacturing considerations, design of biocontainment facility and process based laboratory, and sustainability considerations, as well as an outlook on the facility of the future. Provides guidelines for meeting licensing and regulatory requirements for biomanufacturing facilities in the U.S.A and WHO—especially in emerging global markets in India, China, Latin America, and the Asia/Pacific regions Focuses on innovative design and equipment, to speed construction and time to market, increase energy efficiency, and reduce footprint, construction and operational costs, as well as the financial risks associated with construction of a new facility prior to the approval of the manufactured products by regulatory agencies Includes many diagrams that clarify the design approach Process Architecture in Biomanufacturing Facility Design is an ideal text for professionals involved in the design of facilities for manufacturing of biopharmaceuticals and vaccines, biotechnology, and life-science industry, including architects and designers of industrial facilities, construction, equipment vendors, and mechanical engineers. It is also recommended for university instructors, advanced undergraduates, and graduate students in architecture, industrial engineering, mechanical engineering, industrial design, and industrial interior design.