Friedrich Lottspeich

Bioanalytics

Friedrich Lottspeich

Analytical methods are the essential enabling tools of the modern biosciences. This book presents a comprehensive introduction into these analytical methods, including their physical and chemical backgrounds, as well as a discussion of the strengths and weakness of each method. It covers all major techniques for the determination and experimental analysis of biological macromolecules, including proteins, carbohydrates, lipids and nucleic acids. The presentation includes frequent cross-references in order to highlight the many connections between different techniques. The book provides a bird´s eye view of the entire subject and enables the reader to select the most appropriate method for any given bioanalytical challenge. This makes the book a handy resource for students and researchers in setting up and evaluating experimental research. The depth of the analysis and the comprehensive nature of the coverage mean that there is also a great deal of new material, even for experienced experimentalists. The following techniques are covered in detail: – Purification and determination of proteins – Measuring enzymatic activity – Microcalorimetry – Immunoassays, affinity chromatography and other immunological methods – Cross-linking, cleavage, and chemical modification of proteins – Light microscopy, electron microscopy and atomic force microscopy – Chromatographic and electrophoretic techniques – Protein sequence and composition analysis – Mass spectrometry methods – Measuring protein-protein interactions – Biosensors – NMR and EPR of biomolecules – Electron microscopy and X-ray structure analysis – Carbohydrate and lipid analysis – Analysis of posttranslational modifications – Isolation and determination of nucleic acids – DNA hybridization techniques – Polymerase chain reaction techniques – DNA sequence and epigenetic modification analysis – Analysis of protein-nucleic acid interactions – Analysis of sequence data – Proteomics, metabolomics, peptidomics and toponomics – Chemical biology

Microcharacterization of Proteins

Friedrich Lottspeich

Proteomics – the analysis of the whole set of proteins and their functions in a cell – is based on the revolutionary developments which have been achieved in protein analysis during the last years. The number of finished genome projects is growing and in parallel there is a dramatically increasing need to identify the products of revealed genes. Acting on a micro level modern protein chemistry increases our understanding of biological events by elucidating the relevant structure-function relationships. The second edition of the successful title Microcharacterization of Proteins presents a current overview of modern protein analysis: From sample preparation to sequence analysis, mass spectrometry and bioinformatics it informs about the tools needed in protein research. This makes the book indispensable for everyone involved in proteomics!