Principles of Virology, Volume 2. S. Jane Flint

Links to Internet resources such as websites, podcasts, blog posts, and movies are provided within each chapter; the digital edition provides one-click access to these materials.

      As in our previous editions, we have tested ideas for inclusion in the text in our own classes. We have also received constructive comments and suggestions from other virology instructors and their students. Feedback from our readers was particularly useful in finding typographical errors, clarifying confusing or complicated illustrations, and pointing out inconsistencies in content.

      For purposes of readability, references are not included within the text; each chapter ends with an updated list of relevant books, review articles, and selected research papers for readers who wish to pursue specific topics. New to this edition are short descriptions of the key messages from each of the cited papers of special interest. Finally, each volume has a general glossary of essential terms.

      These two volumes outline and illustrate the strategies by which all viruses reproduce, how infections spread within a host, and how they are maintained in populations. We have focused primarily on animal viruses, but have drawn insights from studies of viruses that reproduce in plants, bacteria, and archaea.

       Volume I: The Science of Virology and the Molecular Biology of Viruses

This volume examines the molecular processes that take place in an infected host cell. Chapter 1 provides a general introduction and historical perspective, and includes descriptions of the unique properties of viruses. The unifying principles that are the foundations of virology, including the concept of a common strategy for viral propagation, are then described. The principles of the infectious cycle, descriptions of the basic techniques for cultivating and assaying viruses, and the concept of the single-step growth cycle are presented in Chapter 2.

      The fundamentals of viral genomes and genetics, and an overview of the surprisingly limited repertoire of viral strategies for genome replication and mRNA synthesis, are topics of Chapter 3. The architecture of extracellular virus particles in the context of providing both protection and delivery of the viral genome in a single vehicle is considered in Chapter 4. Chapters 5 to 13 address the broad spectrum of molecular processes that characterize the common steps of the reproductive cycle of viruses in a single cell, from decoding genetic information to genome replication and production of progeny virions. We describe how these common steps are accomplished in cells infected by diverse but representative viruses, while emphasizing common principles. Volume I concludes with a chapter that presents an integrated description of cellular responses to illustrate the marked, and generally irreversible, impact of virus infection on the host cell.

      The appendix in Volume I provides concise illustrations of viral reproductive cycles for members of the main virus families discussed in the text. It is intended to be a reference resource when reading individual chapters and a convenient visual means by which specific topics may be related to the overall infectious cycles of the selected viruses.

       Volume II: Pathogenesis, Control, and Evolution

      This volume addresses the interplay between viruses and their host organisms. In Chapter 1, we introduce the discipline of epidemiology, and consider basic aspects that govern how the susceptibility of a population is controlled and measured. Physiological barriers to virus infections, and how viruses spread in a host, and to other hosts, are the topics of Chapter 2. The early host response to infection, comprising cell-autonomous (intrinsic) and innate immune responses, are the topics of Chapter 3, while the next chapter considers adaptive immune defenses, which are tailored to the pathogen, and immune memory. Chapter 5 focuses on the classical patterns of virus infection within cells and hosts, and the myriad ways that viruses cause illness. In Chapter 6, we discuss virus infections that transform cells in culture and promote oncogenesis (the formation of tumors) in animals. Next, we consider the principles underlying treatment and control of infection. Chapter 7 focuses on vaccines, and Chapter 8 discusses the approaches and challenges of antiviral drug discovery. In Chapter 9, the new chapter in this edition, we describe the rapidly expanding applications of viruses as therapeutic agents. The origin of viruses, the drivers of viral evolution, and host-virus conflicts are the subjects of Chapter 10. The principles of emerging virus infections, and humankind’s experiences with epidemic and pandemic viral infections, are considered in Chapter 11. Chapter 12 is devoted entirely to the “AIDS virus,” human immunodeficiency virus type 1, not only because it is the causative agent of the most serious current worldwide epidemic but also because of its unique and informative interactions with the human immune defenses. Volume II ends with a chapter on unusual infectious agents, viroids, satellites, and prions.

      The Appendix of Volume II affords snapshots of the pathogenesis of common human viruses. This appendix has been completely re-envisioned in this edition, and now includes panels that define pathogenesis, vaccine and antiviral options, and the course of the infection through the human body. This consistent format should allow students to find information more easily, and compare properties of the selected viruses.

For some behind-the-scenes information about how the authors created the previous edition of Principles of Virology, see: http://bit.ly/Virology_MakingOf.

Acknowledgments

      These two volumes of Principles could not have been composed and revised without help and contributions from many individuals. We are most grateful for the continuing encouragement from our colleagues in virology and the students who use the text. Our sincere thanks also go to colleagues who have taken considerable time and effort to review the text in its evolving manifestations. Their expert knowledge and advice on issues ranging from teaching virology to organization of individual chapters and style were invaluable and are inextricably woven into the final form of the book.

      We also are grateful to those who gave so generously of their time to serve as expert reviewers of individual chapters or specific topics in these two volumes: Siddharth Balachandran (Fox Chase Cancer Center), Paul Bieniasz (Rockefeller University), Christoph Seeger (Fox Chase Cancer Center), and Laura Steel (Drexel University College of Medicine). Their rapid responses to our requests for details and checks on accuracy, as well as their assistance in simplifying complex concepts, were invaluable.

      As noted in “What’s New,” we benefited from the efforts of the students and postdoctoral fellows who provided critiques on our chapters and helped to guide our revisions: Pradeep Morris Ambrose, Ruchita Balasubramanian, Mariana Nogueira Batista, Pierre Michel Jean Beltran, Marni S. Crow, Qiang Ding, Florian Douam, Jenna M. Gaska, Laura J. Halsey, Eliana Jacobson, Orkide O. Koyuncu, Robert LeDesma, Rebecca Markham, Alexa McIntyre, Katelynn A. Milora, Laura A. M. Nerger, Morgan Pantuck, Chen Peng, Katrien Poelaert, Daniel Poston, Anagha Prasanna, Pavithran T. Ravindran, Inna Ricardo-Lax, Fabian Schmidt, Andreas Solomos,