Principles of Virology, Volume 1. Jane Flint

two-step growth curves of bacteriophages. Growth of a bacteriophage in E. coli under conditions when all cells are infected (left) and when only a few cells are infected (right).

      The results of these experiments defined two new terms in virology: the eclipse period, the phase in which infectivity is lost when virions are disassembled after penetrating cells, and the latent period, the time it takes to replicate and assemble new virus particles before lysis, ∼20 to 25 min for some E. coli bacteriophages.

Synchronous infection, the key to the one-step growth cycle, is usually accomplished by infecting cells with a sufficient number of virus particles to ensure that most of the cells are infected rapidly. Exactly how many virus particles must be added is described by the multiplicity of infection (Box 2.11).

One-Step Growth Analysis: a Valuable Tool for Studying Animal Viruses

      One-step growth analysis soon became adapted for studying the reproduction of animal viruses. The experiment begins with removal of the medium from the cell monolayer and addition of virus in a small volume to promote rapid adsorption. After ∼1 h, unadsorbed inoculum containing virus particles is removed, the cells are washed, and fresh medium is added. At different times after infection, samples of the cell culture supernatant are collected and the virus titer is determined. The kinetics of intracellular virus production can be monitored by removing the medium containing extracellular particles, scraping the cells into fresh medium, and lysing them. A cell extract is prepared after removal of cellular debris by centrifugation, and the virus titer in the extract is measured.

The results of a one-step growth experiment establish a number of important features about viral reproduction. In the example shown in Fig. 2.19A, the first 11 h after infection constitute the eclipse period, during which the viral nucleic acid is uncoated from its protective shell and no infectious virus can be detected inside cells. The small number of infectious particles detected during this period probably represents adsorbed virus that was not uncoated. Beginning at 12 h after adsorption, the quantity of intracellular infectious virus particles begins to increase, marking the onset of the synthetic phase, during which assembly begins. During the latent period, no extracellular virus can be detected. At 18 h after adsorption, virions are released from cells into the extracellular medium. Ultimately, virus numbers plateau as the cells become metabolically and structurally incapable of supporting additional reproduction.

BOX 2.11

      DISCUSSION

       Multiplicity of infection (MOI)

      Infection depends on the random collision of cells and virus particles. When susceptible cells are mixed with a suspension of virus particles, some cells are uninfected and other cells receive one, two, three, etc., particles. The distribution of virus particles per cell is best described by the Poisson distribution:

      P(k) = e^–mmk/k!

      In this equation, P(k) is the fraction of cells infected by k virus particles. The multiplicity of infection, m, is calculated from the proportion of uninfected cells, P(0), which can be determined experimentally. If k is made 0 in the above equation, then

      P(0) = e^–m and m = −1n P(0)

      The fraction of cells receiving 0, 1, and >1 virus particle in a culture of 10⁶ cells infected with an MOI of 10 can be determined as follows.

      The fraction of cells that receive 0 particles is

      P(0) = e–10 = 4.5 × 10^–5

      and in a culture of 10⁶ cells, this equals 45 uninfected cells.

      The fraction of cells that receive 1 particle is

      P(1) = 10 × 4.5 × 10^–5 = 4.5 × 10^–4

      and in a culture of 10⁶ cells, 450 cells receive 1 particle.

      The fraction of cells that receive >1 particle is

      P(>1) = 1 − e^–m(m + 1) = 0.9995

      and in a culture of 10⁶ cells, 999,500 cells receive >1 particle. [The value in this equation is obtained by subtracting from 1 (the sum of all probabilities for any value of k) the probabilities P(0) and P(1).]

      The fraction of cells receiving 0, 1, and >1 virus particle in a culture of 10⁶ cells infected with an MOI of 0.001 is

      1 P(0) = 99.99%P(1) = 0.0999% (for 106 cells, 104 are infected)P(>1) = 10–6The MOI required to infect 99% of the cells in a cell culture dish isP(0) = 1% = 0.01m = −1n (0.01) = 4.6 PFU per cell.

      The yield of infectious virus per cell can be calculated from the data collected during a one-step growth experiment. This value varies widely among different viruses and with different virus-host cell combinations. For many viruses, increasing the multiplicity of infection above a certain point does not increase the yield: cells have a finite capacity to produce new virus particles. In fact, infecting at a very high multiplicity of infection can cause premature cell lysis and decrease virus yields.

      The kinetics of the one-step infectious cycle can vary dramatically among viruses. For example, enveloped viruses that mature by budding from the plasma membrane, as discussed in Chapter 13, generally become infectious only as they leave the cell, and therefore, little intracellular infectious virus can be detected (Fig. 2.19B). The curve shown in Fig. 2.19A illustrates the pattern observed for a DNA virus with the long latent and synthetic phases typical of many DNA viruses, some retroviruses, and reovirus. For small RNA viruses, the entire growth curve is complete within 6 to 8 h, and the latent and synthetic phases are correspondingly shorter.

      One-step growth curve analysis can provide quantitative information about different virus-host systems. It is frequently employed to study mutant viruses to determine what parts of the infectious cycle are affected by a particular genetic lesion. It is also valuable for studying the multiplication of a new virus or viral reproduction in a new virus-host cell combination.

      When cells are infected at a low multiplicity of infection, several cycles of viral reproduction may occur (Fig. 2.18B). Growth curves established under these conditions can also provide useful information. When infection is carried out at a high multiplicity, a mutation may fail to have an obvious effect on viral reproduction. The defect may only become evident following a low-multiplicity infection. Because the effect of a mutation in each cycle is multiplied, a small effect can be amplified after several cycles. Defects in the ability of viruses to spread from cell to cell may also be revealed when multiple cycles of reproduction occur.

Global Analysis

      The study of replication cycles of many viruses with one-step growth analysis has allowed a reductionist approach to understanding and defining the steps of virus attachment, entry, replication, and assembly. In contrast, new experimental and computational tools permit global analysis of viral, cellular, and host responses to infection. Global analyses apply a dizzying array of different high-throughput technologies to measure system-wide changes in DNA, RNA, proteins, and metabolites during virus infection of cells,