Analytical Food Microbiology. Ahmed E. Yousef

using the mesophilic ...

      5 Chapter 6Figure 6.1 Life cycle of a spore‐forming bacterium. Dark shades (e.g., cell ...Figure 6.2 Transmission electron micrograph of Bacillus subtilis spores (Cou...Figure 6.3 Outline of procedure for counting aerobic and anaerobic mesophili...

      6 Chapter 7Figure 7.1 Scheme of testing food for Pseudomonas spp. and other psychrotrop...

      7 Chapter 8Figure 8.1 Detection and enumeration of Enterobacteriaceae in food.Figure 8.2 Most‐probable number (MPN) Enterobacteriaceae enrichment broth (E...

      8 Chapter 9Figure 9.1 Classification of fungi associated with food. Some genera are gro...Figure 9.2 Scheme of testing food for fungi.Figure 9.3 Slide culture assemblyFigure 9.4 Hand‐held hard cheese shredder.

      9 Chapter 10Figure 10.1 Enumeration and identification of Staphylococcus aureus in food....Figure 10.2 Colonies of Staphylococcus aureus (a) and S. epidermidis (b) on ...Figure 10.3 Detection of staphylococcal enterotoxin gene (sea) in food isola...Figure 10.4 Basics of gene expression in bacterial cell.Figure 10.5 Enzyme‐linked immunoassay used in detection of staphylococcal en...Figure 10.6 Microfiltration to prepare cell‐free culture supernatant.

      10 Chapter 11Figure 11.1 Detection of Listeria spp. in food and environmental sample usin...Figure 11.2 Detection of Listeria monocytogenes in food and environmental sa...

      11 Chapter 12Figure 12.1 Method for detection of Salmonella in food.Figure 12.2 Scoring the incubated API‐20E strip for food isolate and positiv...

      12 Chapter 13Figure 13.1 Complete procedure for the detection of Shiga toxin‐producing Es...Figure 13.2 Detection of Shiga toxin‐producing Escherichia coli (STEC) in fo...Figure 13.3 Detection of Shiga toxin‐producing Escherichia coli in food samp...Figure 13.4 Gel showing multiplex PCR products resulting from Escherichia co...Figure 13.5 Matrix of 24 inculcated microfuge tubes, prepared for PCR‐assist...

      13 Chapter 14Figure 14.1 Illustration of a survivor plot of an organism.Figure 14.2 Illustration of a thermal resistance plot for an organism.Figure 14.3 Procedure to determine thermotolerance of inoculated bacteria in...Figure 14.4 Linear‐linear graphing paper.

      14 Chapter 15Figure 15.1 Bioassay for quantifying antimicrobial activity in a fermentate ...Figure 15.2 Bioassay for quantifying antimicrobial activity in milk fermenta...Figure 15.3 Inhibition areas resulting from spotting dilutions of nisin stoc...Figure 15.4 Dose response plot depicting the linear relationship between logFigure 15.5 Procedure overview of production of antimicrobial ingredients.Figure 15.6 Microfiltration of bacteriocin‐containing fermentate.

      15 Part 2Figure II.1 Typical links in the food supply chain.Figure II.2 Taxonomy of foodborne bacteria, drawn from Bergey’s manual of sy...

      16 Part 3Figure III.1 Enzyme‐linked immunosorbent bioassay (ELISA) technique for dete...Figure III.2 Polymerase chain reaction (PCR) method for amplifying unique DN...

      Guide

      1  COVER PAGE

      2  TITLE PAGE

      3  COPYRIGHT PAGE

      4  PREFACE

      5  TABLE OF CONTENTS

      6  BEGIN READING

      7  APPENDIX I LABORATORY EXERCISE REPORT

      8  APPENDIX II BACTERIAL AND FUNGAL STRAINS RECOMMENDED FOR USE AS CONTROL ORGANISMS

      9  APPENDIX III MICROBIOLOGICAL MEDIA

      10  APPENDIX IV SUPPLIES AND EQUIPMENT AVAILABILITY

      11  INDEX

      12  WILEY END USER LICENSE AGREEMENT

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