Pathology of Genetically Engineered and Other Mutant Mice. Группа авторов

disorganized with less clear separation of B and T cell areas [55]. B cell follicles are reduced in size and there is a reduction in the number and function of follicular dendritic cells. The marginal zone and metallophilic marginal zone macrophages no longer form a continuous cell layer [55, 56].

       Amyloidosis: Amyloid in the spleen is usually AA‐amyloid associated with chronic inflammation. Experimental models indicate that AA amyloid first accumulates in the marginal zone and is associated with loss of marginal zone macrophages [57]. As the amyloidosis increases in severity, deposits expand into the red pulp.

       Pigment: The appearance of hemosiderin pigment is normal in the red pulp of the spleen. Red pulp macrophages are engaged in erythrophagocytosis and frequently contain hemosiderin. The amount of hemosiderin is greater in female mice, but varies by mouse strain. Hemosiderin can be distinguished from other pigments by Prussian blue staining (Figure 7.7). Lipofuscin‐ceroid pigment may be observed in red pulp macrophages, but is less common than hemosiderin. In mice with black fur, the dorsal one‐third of the spleen is often dark red to black as a result of accumulation of melanin. Light microscopically, dendritic melanocytes and melanin‐laden macrophages are present in the red pulp, and occasionally in the white pulp (Figure 7.7).Figure 7.6 Spleen. Spleen of C57BL/6J (a), B6.Cg‐Foxn1nu (b), B6.129S7‐Rag1tm1Mom (c), and NOD.Cg‐Prkdcscid Il2rgtm1Wjl/SzJ (NSG) mice (d).

       Increased extramedullary hematopoiesis (EMH): While modest EMH is normal in the red pulp of the spleen of adult mice, increased EMH can lead to marked expansion of the red pulp and is a common cause of splenomegaly. The myeloid lineage predominates when there is inflammation in other organs, while hemorrhage and red blood cell destruction may lead to an increase of erythrocyte precursors and sometimes megakaryocytes.

       Red pulp atrophy and hypoplasia: A reduced size of the red pulp may be seen in mice with blocks in cell division as a result of genetic mutations or chemical treatment.

      Milky Spots

      Milky spots are collections of leukocytes located just beneath the mesothelium in the omentum [58]. They are predominantly composed of macrophages and B1 B cells with fewer classical B2 B cells. They contain few T cells and lack a distinct T cell area that is present in other secondary lymphoid organs. Furthermore, follicular dendritic cells are absent from milky spots. Lymphoid structures similar to milky spots are found in pericardial, mediastinal, and mesenteric fat, and these are known as fat‐associated lymphoid clusters (FALCs) [59]. The formation of milky spots and FALCs requires LTA and CXCL13, but is independent of lymphoid tissue‐inducer cells. The number and size of milky spots and FALCs is increased by exposure to microbiota, suggesting that these tissues have features in common with both secondary and tertiary lymphoid tissues. Milky spots lack afferent lymphatics, and antigens enter via fenestrations in the overlying mesothelium. Inflammation and infections in the peritoneal cavity lead to a rapid increase of the number and size of milky spots as a result of an influx of peritoneal macrophages and recruitment of cells from the blood circulation. Immune responses can involve both B1 cells which differentiate into IgM and IgA‐secreting plasma cells and conventional B and T cells.

      Tertiary Lymphoid Organs

Organized lymphoid tissues can form at sites of chronic inflammation associated with autoimmune diseases, infections, and neoplasms (Figure 7.8). Immunohistochemistry can identify distinct T and B cell areas with high‐endothelial venules, follicles with follicular dendritic cells and sometimes germinal centers. The same cytokines and chemokines that orchestrate the formation of secondary lymphoid organs play a role, but tertiary lymphoid organs are formed after birth and not at preselected sites [33]. The role of tertiary lymphoid organs in inflammation and cancer is not well established. Tertiary lymphoid organs associated with autoimmune inflammation are thought to contribute to the autoimmune response and be detrimental. However, tertiary lymphoid organs formed in the adventitial layer of the aorta in ApoE‐deficient mice (B6.129P2‐Apoe^tm1Unc/J) with atherosclerosis seem to have a beneficial effect as the atherosclerosis was more severe in mice in which the formation of the serosal lymphoid tissue was blocked [60]. The presence of tertiary lymphoid organs in and near tumors is thought to increase the response to immunotherapy and to be a positive prognostic indicator [61].

Figure 7.7 Melanin vs. hemosiderin. (a) Spleen of B6.Cg‐Prkdc^scid/SzJ mouse with melanocytes and melanin‐laden macrophages and hemosiderin. (b) Prussian blue stain distinguishes hemosiderin (blue) and melanin (dark brown) pigments.

Figure 7.8 Tertiary lymphoid structures. Tertiary lymphoid tissue with two follicles with germinal centers in the kidney of a CD‐1 mouse with chronic pyelonephritis.

Acknowledgments

      This work was supported grants from the National Institutes of Health (R01‐CA089713 and P30‐CA034196 to JPS).

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