Pathology of Genetically Engineered and Other Mutant Mice. Группа авторов

has been an amazing opportunity to follow the development of mouse models from the earliest days of spontaneous mutations occurring in mouse colonies, induced mutations using radiation or chemical mutagenesis, to the introduction of transgenic technology, then targeted mutagenesis (knock‐out mouse technology), to CRISPR/Cas9, and beyond. Parallel to the advances in molecular technology has been refinement of histopathological approaches beyond H&E and special stained sections to immunofluorescence, immunohistochemistry, in situ hybridization, and transcriptome analysis on organs and now to single cells. Despite all these advances, histopathology will always be a backbone for characterizing the biology and pathology of animals including humans. We have seen refinements on all fronts resulting in excellent models for human diseases for dissecting molecular mechanisms to running preclinical trials. This book reflects the current state of the art in pathology phenotyping of mouse models, and we hope will lay the groundwork for many discoveries yet to come.

1 Introduction to Mouse Pathology

       Jerrold M. Ward, Peter Vogel, and John P. Sundberg

The Use of Mice in Medical Research

      There has been a large increase in the use of mice in both basic and translational medical research over the past 20 years, as evidenced by the increase in retrievable references using “mice” as a keyword in PubMed from 29 029 publications in 1998 to 79 858 in 2018, and over 1.6 million in the entire PubMed database. Mice have been used for both basic and translational (formerly applied) research. Much of this important work is sponsored by the National Institutes of Health (NIH) in the United States. Mice have been used to study basic biological processes, embryo development, genetic disorders, infectious diseases, degenerative diseases, toxicology, carcinogenesis, and aging, often by organ system or tissue. Many Nobel Prize awardees in Physiology or Medicine have used mice in research leading to their honors. Much of this research has been greatly valued for the training of future scientists, discovery of new diseases, understanding the mechanism of disease in mice and other animals, including humans, and in the treatment and prevention of disease in mice and humans [1–8]. Histopathology may be included in the mouse research, but often it is not, or not by someone trained and competent in pathology [9]. The value of pathology has been proven for diagnosis and understanding normal biology and abnormal biology (pathology) of cells, tissues, and organs in all species. Comparative pathology spans all species of animals. But some investigators do not understand the value of pathology, as a discipline, in experimental studies with mice. This book intends to promote the value of mouse pathology in medical research aimed at the discovery of the causes, prevention, and therapy of diseases in both humans and other animals.

Understanding Diseases Found in Mutant Animals

Naturally occurring and induced changes in genes often result in a specific phenotype at the clinical and histopathological levels. The cause of this phenomenon is that genes have specific functions that play a role in the normal homeostasis of cells, tissues, and organs. A genetically engineered mouse () line represents a tool to investigate the effects resulting from the partial or complete loss of gene function or the gain of normal or abnormal functions. The genes are often found to function in specific cellular organelles and biochemical/molecular pathways important for normal biological functions (Figure 1.1) [10]. Cells involved in common gene functions can be single cell types in single tissues, multiple cells types in a single tissue, or multiple cells types in multiple tissues. Gene expression can also be induced in specific cells and tissues by normal and abnormal body functions, or by exposure to external factors such as drugs, infectious agents, environmental modifications, and ingested foods.

Mutant mice often exhibit histopathological changes (lesions) in tissues and organs that are associated with gene function, but the ultimate clinical phenotype can be influenced by various factors. Much of the histopathology found in mutant mice is in the usual spectrum of degenerative, inflammatory, proliferative, and neoplastic changes also found in nonmutant mice. Nouvelle lesions do occur commonly in some lines of mutant mice. These include unique developmental changes, cellular morphological changes, patterns of lesions, types of proliferative lesions, and often strain specific cancer types. These may be due to specific naturally occurring mutations (polymorphisms) in genes that can serve as genetic based models for disease. Spontaneous mutants made up the bulk of mouse models until the advent first of radiation and then chemical mutagenesis programs. The spectrum of spontaneous lesions is noted in each organ‐specific chapter. Often the lesions are identical or very similar to those associated human genetic disorders, but they also may vary from human lesions. The genetic background of the mice often plays a role in spontaneous as well as induced disease phenotypes.

Figure 1.1 Classes of proteins associated with human genetic diseases.

      Source: Nussbaum (2007). Reprinted with permission of Elsevier

Mouse Pathology – Nomenclature

The pathology of mice in research was first led by Thelma Dunn and Harold Stewart at the National Cancer Institute (NCI) at the NIH [11–13]. Both were MD pathologists who applied general rules of human pathology diagnosis to mice when possible. Although mouse pathology nomenclature does not follow any official designation, two organizations (INHAND; https://www.toxpath.org/inhand.asp and National Cancer Institute Mouse Models of Cancer Consortium Tumor Pathology Nomenclature) have provided international nomenclatures for specific tissues [2, 14]. Other published guides are included in each appropriate organ chapter [15, 16]. Many books and refereed publications on mouse pathology provide valuable information for pathologists and scientists [12,17–35] as well as web sites (https://ntp.niehs.nih.gov/nnl; http://www.informatics.jax.org/frithbook; http://eulep.pdn.cam.ac.uk/~skinbase/index.php). Importantly, many of these references involve both DVMs and MDs, pathologists, and basic scientists, who integrate mouse and human disease nomenclature together to be state‐of‐the‐art. The pathology nomenclature used in this book generally reflects the NCI tumor pathology and INHAND general pathology nomenclatures. There are, however, no international or national standards for nomenclature that must be followed. These published nomenclatures are merely guidelines for use by scientists, pathologists, and journals. Each chapter author considered these guidelines and noted appropriate references for each organ and tissue.

Mouse Genetic Nomenclature

      In contrast to pathology nomenclature, mouse genetic nomenclature is standardized. Chapter 3 focuses on the details of the nomenclature system and discusses how it was developed. While the authors and editors have, for the most part, updated the nomenclature, not all authors were willing to do so. Regardless, one can and should use the Mouse Genome Informatics website to verify all genes and alleles, as discussed in the Chapter 3, to make sure they are working with the correct nomenclature and allelic mutations.

Tumor Pathology

      It is known in human and mouse pathology that cancer pathogenesis follows a scheme of molecular pathogenesis and an associated histopathogenesis [14, 16, 36]. There have been numerous publications on the role of specific genes in tumor pathogenesis in humans and animals. It is not the intention of this book to review the role of all genes for which published information on mouse cancer models is available,