Analysieren von Proben – preiswert und effektiv Die elektrochemische Stripping-Analyse stellt eine au?erst empfindliche und gleichzeitig einfache und preiswerte Analysenmethode dar. Von einer kurzen theoretischen Einfuhrung uber den Aufbau einfacher Me?anordnungen bis hin zu zahlreichen Anwendungsmoglichkeiten bietet dieses Buch alles Wichtige uber diese Methode. Der Schwerpunkt liegt auf den praktischen Aspekten und den Anwendungsbeispielen aus der Spuren- und Umweltanalytik. Dabei gibt der Autor dem Leser Hilfen zum Auffinden der fur seine Probleme geeigneten Me?methode und weist auf Stormoglichkeiten bei den verschiedenen Messungen hin. Die ausfuhrliche Beschreibung der Instrumentation versetzt den Benutzer in die Lage, einfache Me?anordnungen auf seine Bedurfnisse zugeschnitten selbst zusammenzubauen. Dieses Buch ist fur alle Praktiker der Analytik, wie Chemieingenieure und Chemotechniker, aber ebenso fur Praktikumsleiter in Ausbildungsstatten geeignet. Der Autor war 25 Jahre auf dem Gebiet der Spuren- und Umweltanalytik bei Siemens tatig.
Neben der Methodenentwicklung ist die Optimierung bestehender Methoden eine zentrale Aufgabe im HPLC-Labor. Eine Aufgabe, die heute in immer kurzerer Zeit und kosteneffizient erledigt werden muss. Das Handbuch bietet eine fundierte Hilfe, um diese Herausforderung noch besser zu meistern. International renommierte Autoren wie John W. Dolan, Michael McBrien, Veronika R. Meyer, Uwe D. Neue, Lloyd R. Snyder oder Klaus K. Unger behandeln sowohl die allgemeinen Grundlagen und Strategien der Optimierung als auch die spezifischen Aspekte der unterschiedlichen Techniken wie RP-HPLC, NP-HPLC, Micro- und Nano-HPLC sowie der Kopplungstechniken wie LC-MS. Auch die richtige Saulenauswahl sowie Enantiomerentrennungen gehoren zu den behandelten Themen. Die Autoren liefern konkrete, praktische Tipps ebenso wie relevante Hintergrundinformationen. Sie bieten daruber hinaus Einblicke in die Optimierungspraxis sieben international renommierter Firmen verschiedener Branchen. Einige Beitrage stellen die Anwendung gangiger Optimierungssoftware wie DryLab oder ChromSword dar. Das ganze wird abgerundet durch praxisnahe Berichte erfahrener Anwender aus den verschiedenen Anwendungsgebieten, inbesondere aus den Life Sciences, wie beispielsweise Proteomics oder Pharmaentwicklung. Alle Beitrage sind in einem auf das Wesentliche konzentrierten und anwendungsnahen Stil geschrieben. Der Aufbau des Buches mit abgeschlossenen Kapiteln erleichtert das gezielte Nachschlagen.
This comprehensive reference and handbook covers all aspects of ultrasound for analytical applications. Besides classical extraction techniques, it also provides an overview of ultrasound applications and devotes two chapters to proteomics and polymer technology. From the contents: * Common ultrasonic devices * Elemental speciation * On-line applications * Accelerated extraction of semivolatile and volatile organics * The ultrasonic bath vs. the ultrasonic probe * Liquid-liquid, liquid-solid and solid-liquid extraction * Solid-phase (micro)extraction * Stir bar sorptive extraction * Sonochemistry for organic and inorganic synthesis * Electrochemical applications * Applications to polymer science * Power ultrasound meets proteomics Of great interest to researchers in academia and industry, as well as analytical and natural products chemists, and those working in trace analysis.
Thoroughly revised, with either entirely new or completely updated contents, this is a practical manual for the small and large-scale preparation of enantiomerically pure products. The result is a vital resource for meeting the highest purity standards in the manufacture of chiral pharmaceuticals, food additives and related compounds. All the approaches covered here are highly relevant to modern manufacturing and quality control schemes in the pharmaceutical and biotech industries, addressing the increasingly important issue of drug safety in view of tougher regulatory standards worldwide.
The use of light-emitting proteins for the detection of biomolecules provides fast and sensitive methods which overcome the disadvantages of radioactive labels and the high cost of fluorescent dyes. This reference work summarizes modern advanced techniques and their applications and includes practical examples of assays based on photoproteins. The book presents contemporary key topics like luminescent marine organisms, DNA probes, reporter gene assays and photoproteins, ratiometric sensing, use of photoproteins for in vivo functional imaging and luminescent proteins in binding assays, to name just a few, and is complemented by recent advances in instrumentation. Includes an introductory chapter by 2008 Chemistry Nobel laureate Osamu Shimomura.
This handbook provides a straightforward introduction to spectroscopy, showing what it can do and how it does it, together with a clear, integrated and objective account of the wealth of information that can be derived from spectra. The sequence of chapters covers a wide range of the electromagnetic spectrum, and the physical processes involved, from nuclear phenomena to molecular rotation processes. – A day-by-day laboratory guide: its design based on practical knowledge of spectroscopists at universities, industries and research institutes – A well-structured information source containing methods and applications sections framed by sections on general topics – Guides users to a decision about which spectroscopic method and which instrumentation will be the most appropriate to solve their own practical problem – Rapid access to essential information – Correct analysis of a huge number of measured spectra data and smart use of such information sources as databases and spectra libraries
This laboratory guide for successful electrophoretic separations is divided into two parts to provide readers with a thorough presentation of the fundamentals followed by a detailed description of the most common methods currently in use. This fourth edition retains the successful concept of its predecessors, yet features a brand-new layout, and is further enhanced by a section on difference gel electrophoresis, while the chapter on proteome analysis is practically all new and considerably extended, plus there are now around 10 % new literature references.
As analytical chemistry and biology move closer together, biologists are performing increasingly sophisticated analytical techniques on cells. Chemists are also turning to cells as a relevant and important sample to study newly developed methods. Practical Cell Analysis provides techniques, hints, and time-saving tips explaining what may be “common knowledge” to one field but are often hidden or unknown to another. Within this practical guide: The procedures and protocols for cell separation, handling cells on a microscope and for using cells in microfluidic devices are presented. Elements of cell culture are taken and combined with the practical advice necessary to maintain a cell lab and to handle cells properly during an analysis The main chapters deal with the fundamentals and applied aspects of each technique, with one complete chapter focusing on statistical considerations of analyzing cells Many diagram-based protocols for some of the more common cell processes are included Chapter summaries and extensive tables are included so that key information can be looked up easily in the lab setting Much like a good manual or cookbook this book is a useful, practical guide and a handy reference for all students, researchers and practitioners involved in cellular analysis.
Easy-to-follow guide helps you take full advantage of EPR spectroscopy's capabilities Electron Paramagnetic Resonance: A Practitioner's Toolkit serves as a practical guide that enables you to navigate through and make sense of the complex maze of electron paramagnetic resonance (EPR) spectroscopy fundamentals, techniques, and applications. The first half of this book is dedicated to explaining the core principles of EPR spectroscopy, using clear, easy-to-follow explanations and examples while avoiding complex physics and mathematics. The second half of the book focuses on applications, including problem-solving strategies for such fields as biology, medicine, material science, chemistry, physics, and radiation effects on matter. Carefully edited by two experienced EPR scientists, this book features a team of eighteen expert authors. Their contributions are based not only on a thorough examination and analysis of the primary literature, but also on their own firsthand experience in research and applications. As a result, the book is filled with practical advice, tips, and cautions addressing such issues as: Choosing the right experiment Selecting experimental parameters and sample size Avoiding setbacks and pitfalls Simulating the spectra With its straightforward explanations and clear examples, this book is just what researchers need to take full advantage of EPR spectroscopy's tremendous capabilities. It is particularly recommended for those interested in applications to chemistry, biology, medicine, and material science.
Multidimensional Liquid Chromatography (MDLC) is a very powerful separation technique for analyzing exceptionally complex samples in one step. This authoritative reference presents a number of recent contributions that help define the current art and science of MDLC. Topics covered include instrumentation, theory, methods development, and applications of MDLC in the life sciences and in industrial chemistry. With the information to help you perform very difficult separations of complex samples, this reference includes chapters contributed by leading experts or teams of experts.